Composite
Part:BBa_K4613025:Design
Designed by: Yuchen Zhou Group: iGEM23_NAU-CHINA (2023-10-10)
pQE-80L-C3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1081
Illegal BamHI site found at 1026 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1191
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 94
Design Notes
We attached 6*his tag to the N-terminus of C3 to get purified C3.
Source
NAU-CHINA 2023