Composite

Part:BBa_K4613025:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


pQE-80L-C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1081
    Illegal BamHI site found at 1026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 94


Design Notes

We attached 6*his tag to the N-terminus of C3 to get purified C3.


Source

NAU-CHINA 2023

References